‘Premier Russet’ has a late vine maturity and ‘Rio Grande Russet’ has a medium late maturity. 0–6.5) than ‘Premier Russet’ (6.0–7.5). For the progeny, mean trait values displayed a wide range of phenotypic variation (Additional file 4: Table S3), with a high correlation between years (> 0.70, P < 0.001) and across environments (> 0.55, P < 0.001). The broad-sense heritability for both years at each location was statistically significant, with H 2 estimates ranging from 0.83 to 0.93 (Table 2). These results were consistent for all vine maturity traits. When mean values for maturity were mapped to the linkage maps, a significant association was detected on chromosome 5 (Fig. 2, Additional file 6: Figure S2). This association was consistent between years and environments. Logarithm of the odds (LOD) scores ranged from of 21.2 and 34.1, well above the permutation thresholds. Collectively, they explained between 38 and 54% of the trait variance (Table 3). The peak of the LOD profile was consistently detected at 15.0 cM (Table 3, Fig. 2). The best simple model for maturity was a double-simplex configuration (AAAB ? AAAB) on homologous chromosomes H2 and H8 of Rio Grande russet (RGR) and Premier Russet (PR), respectively (Additional file 6: Figure S2). This model implies a QTL with allele effects from both parents, and with the B allele associated with earlier maturity (P < 0.001). Thus, progeny with two copies of the B allele (AABB) had on average earlier maturity (3.1–4.0) than progeny with a single copy (AAAB, 4.5–6.2) or lacking the B allele (AAAA, 6.4–7.5).
LOD get distribution out of QTLs to have qualities in the readiness locus part on the chromosome 5. Per committee in the shape is short for a special characteristic. LOD tolerance 95% trust intervals is listed in Desk step three. Ca, citric acidic 2010; EB, early blight; FBE, bud-prevent fry color Idaho; GH, growth routine; GLU, glucose; M95, vine readiness from the 95DAP; M120, vine maturity on 120DAP; TSHA, tuber figure; VW, Verticillium wilt; ID, Idaho; NC, North carolina; DAP, weeks once planting; wide variety ten and you may 11 throughout the trait names imply age 2010 and 2011, correspondingly
The fresh new SNP nearest to the QTL to possess readiness that had a beneficial double-simplex configuration are solcap_snp_c2_11605, located at 9.step 1 cM. Regression of the readiness indicate opinions to the genotype for the SNP informed me between 38 and you may 50% of phenotypic variance. The top of the LOD contour (15 cM) is positioned in this a space anywhere between markers one to spanned 8 to 10 cM, for the solcap_snp_c2_11605 located on you to side of the gap. This area harbors the brand new Solanum tuberosum bicycling DOF foundation gene 1 (StCDF1, PGSC0003DMG400018408), which had been said to be a primary regulator out of potato bush maturity . Visual symbolization of your own hereditary and you can real (Mb) distance out of homologous chromosomes H2 and you may H8 exhibited the brand new questioned bend figure and you will fit better that have chromosome design demonstrating a high studies out-of concordance involving the hereditary condition (cM) and you can real location (Mb) (Most file eight: Profile S3).
Several characteristics co-localize in the readiness locus region towards the chromosome 5
Seven faculties also citric acidic (CANC10), gains routine (GHID, GHNC), bud-prevent fry color (FBEID), very early escort Beaumont blight rating (EBID11), tuber glucose (GLUID11), tuber contour (TSHAID), and you will Verticillium wilt rating (VWID11) co-nearby that have vine readiness characteristics (MAT95ID, MAT95NC, MAT120ID, MAT120NC) to your chromosome 5 (Dining table step three, Fig. 2). An educated genetic design for all traits, except GLUID11 and FBEID was a double-simplex impact on H2 and H8 to your SNP, solcap_snp_c2_11605, that have it setting (Desk 3). If the residuals throughout the regression of the many half dozen characteristics to your vine readiness were assessed, zero tall aftereffect of an excellent QTL was discover to have EBID11, TSHAID, and you will GHNC. To own gains habit (GHID) and you will VWID11, a critical QTL try observed as long as remedied to possess readiness during the 95DAP however, wasn’t high at 120DAP. Research from glucose concentrations (GLUID11) presented an excellent LOD out-of step three.nine at the 21 cM, with an excellent QTL effect on H2 and H5. After modifying for readiness, a maximum on the LOD bend to own GLUID11 try noticed just below the 95% permutation threshold.